By Neil Errington, Teuku Iqbalsyah, Andrew J. Doig (auth.), Raphael Guerois, Manuela López de la Paz (eds.)
Recent significant advances in our realizing of modulating protein services has resulted in the improvement of recent tools and algorithms to foretell and decipher how amino acid sequences form 3-dimensional buildings. Protein layout: tools and purposes provides the main updated protein layout and engineering ideas in order that readers can adopt their very own initiatives with a greatest probability of success.
The authors current built-in computational methods that require quite a few levels of computational complexity, and the foremost accomplishments which were completed within the layout and structural characterization of helical peptides and proteins. different issues of debate comprise: layout of structural effortless motifs, complete proteins, and interfaces of protein complexes, and of amyloidogenic polypeptides and amyloid inhibitors.
Authoritative and state of the art, Protein layout: equipment and purposes can be of significant curiosity to protein scientists, biochemists, and all experimentalists picking the tactic such a lot adaptable for his or her layout problem.
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Extra info for Protein Design: Methods and Applications
Viguera, A. , and Serrano, L. (1998) Elucidating the folding problem of α-helices: local motifs, long-range electrostatics, ionic-strength dependence and prediction of NMR parameters. J. Mol. Biol. 284, 173–191. 54. Muñoz, V. and Serrano, L. (1994) Elucidating the folding problem of helical peptides using empirical parameters. Nat. Struct. Biol. 1, 399–409. 55. Muñoz, V. and L. Serrano, L. (1995) Elucidating the folding problem of helical peptides using empirical parameters. II. Helix macrodipole effects and rational modification of the helical content of natural peptides.
Acad. Sci. USA 90, 11332–11336. 14. , Feliciano, E. , and Nambiar, K. P. (1993) Stabilization of alphahelical structures in short peptides via end capping. Proc. Natl. Acad. Sci. USA 90, 838–842. 15. Dasgupta, S. and Bell, J. A. (1993) Design of helix ends. Amino acid preferences, hydrogen bonding and electrostatic interactions. Int. J. Pept. Prot. Res. 41, 499–511. 16. Harper, E. T. and Rose, G. D. (1993) Helix stop signals in proteins and peptides: the capping box. Biochemistry 32, 7605–7609.
Morozova-Roche, L. , Cochran, D. A. , Doig, A. , and Barron, L. D. (2000) Is polyproline II helix the killer conformation? A Raman optical activity study of the amyloidogenic prefibrillar intermediate of human lysozyme. J. Mol. Biol. 301, 553–563. 103. Barron, L. , Blanch, E. , and Bell, A. F. (2000) Solution structure and dynamics of biomolecules from Raman optical activity. Prog. Biophys. Mol. Biol. 73, 1–49. 26 Errington, Iqbalsyah, and Doig 104. Pace, C. N. and Scholtz, J. M. (1998) A helix propensity scale based on experimental studies of peptides and proteins.