By Selene K. Swanson, Laurence Florens (auth.), Mary S. Lipton, Ljiljana Paša-Tolic (eds.)
When the final variation of this publication was once released in 2000, the sector of proteomics was once in its infancy. Now in its youth, proteomics is essentially remodeling organic and scientific study. a lot of this change may be attributed to technological developments, fairly in mass spectrometry. due to those fast advancements that experience resulted in accelerated components of study and leading edge functions, an replace to Mass Spectrometry of Proteins and Peptides isn't just important, yet arguably vital. Spanning fields from microbial forensics and medical purposes to protein constitution, dynamics and serve as, the subsequent chapters written via best specialists function a discussion board for providing many of the most modern revolutionizing tools for mass spectrometry-based characterization of proteins and peptides. Staying devoted to the spirit of the Methods in Molecular Biology™ sequence, all chapters are written within the related layout, wherein every one bankruptcy opens with a uncomplicated description of the tactic, via an outline of the protocol after which sensible step by step directions to make sure experimental good fortune.
Mass Spectrometry of Proteins and Peptides: equipment and Protocols, moment Editionis a necessary source for beginner and professional practitioners within the fields of biochemistry, molecular biology, medical chemistry, immunology, genetic, microbiology and toxicology.
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Additional info for Mass Spectrometry of Proteins and Peptides: Methods and Protocols
Chen, E. , and Yates, J. , III (2006) Large scale protein profiling by combination of protein fractionation and multidimensional protein 28. 29. 30. 31. 32. 33. 34. 35. 36. 37. 38. identification technology (MudPIT). Mol Cell Proteomics 5, 53-6. Simpson, D. , Mottaz, H. , Vilkov, A. , Anderson, G. , Lipton, M. , and Smith, R. D. (2006) Using size exclusion chromatography-RPLC and RPLC-CIEF as twodimensional separation strategies for protein profiling. Electrophoresis 27, 2722-33. , Sadygov, R. , and Yates, J.
And Yates, J. , III (2006) Large scale protein profiling by combination of protein fractionation and multidimensional protein 28. 29. 30. 31. 32. 33. 34. 35. 36. 37. 38. identification technology (MudPIT). Mol Cell Proteomics 5, 53-6. Simpson, D. , Mottaz, H. , Vilkov, A. , Anderson, G. , Lipton, M. , and Smith, R. D. (2006) Using size exclusion chromatography-RPLC and RPLC-CIEF as twodimensional separation strategies for protein profiling. Electrophoresis 27, 2722-33. , Sadygov, R. , and Yates, J.
Creasy, D. , and Cottrell, J. S. (1999) Probability-based protein identification by searching sequence databases using mass spectrometry data. Electrophoresis 20, 3551-67. , and Stoeckenius, W. (1974) Isolation of the cell membrane of Halobacterium halobium and its fractionation into red and purple membrane. Methods Enzymol 31, 667-78. , and Krippahl, G. (1983) Phototrophic growth of halobacteria and its use for isolation of photosynthetically-deficient mutants. Ann Microbiol (Paris) 134B, 137-50.