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5 Recommendations for Isolation of Fungi from Ocular Specimens RECOMMENDED MEDIA FOR FUNGI ISOLATION Clinical specimens are processed promptly and plated to isolation media as a means to recover fungi that may be causing disease. Media and incubation temperatures are selected to allow for the growth of pathogenic and opportunistic yeasts and fungi. Isolation Media The following Table is intended as a guideline for media required for the primary isolation of common isolates in ocular infections.

A. Profuse growth of hyaline fungus in all C streaks. B. Profuse growth of dematiaceous fungus in all C streaks. C. flavus. D. Mixed growth of fungus and bacteria 7 Identification of Common Ocular Fungal Isolates TECHNIQUES USED FOR MOULD IDENTIFICATION Colony Characteristics • To evaluate colony characteristics of filamentous fungi, it is necessary to subculture the fungus to the same media that the original colony descriptions are based upon. • Visual examination of the colony will rapidly reveal important data concerning color, texture, diffusible pigments, exudates, growth zones, aerial and submerged hyphae, growth rate, colony topography, and macroscopic structures such as ascocarps, pycnidia, sclerotia, sporodochia, and synnemata.

They are ovoid to obclavate, darkly pigmented, muriform, and smooth or roughened. • The end of the conidium nearest the conidiophores is round while it tapers towards the apex. This gives the typical beak or club-like appearance of the conidia. Macroscopic and Microscopic Characteristics of Ocular Fungal Isolates Fig. 3: Alternaria species. Growth on potato dextrose agar Fig. 4: Alternaria species. Obclavate, muriform conidia in chains 47 48 Aravind’s Atlas of Fungal Corneal Ulcers ASPERGILLUS SPECIES Pathogenicity At present, some twenty species of Aspergillus have been recognized as opportunistic pathogens.

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