By Muhammad Sarwar Khan, Iqrar Ahmad Khan, Debmalya Barh
Applied Molecular Biotechnology: the subsequent iteration of Genetic Engineering explains state of the art advances within the quickly constructing sector of molecular biotechnology, the expertise of the recent millennium. created from chapters authored through top specialists of their respective fields, this authoritative reference text:
- Highlights the newest omics-based instruments and techniques utilized in glossy biotechnology
- Explains how a number of molecular biology applied sciences can be utilized to increase transgenic crops and the way these crops can meet starting to be nutrients and plant-derived product demands
- Discusses chloroplast gene expression platforms, mitochondrial omics, plant sensible genomics, and whole-genome resequencing for crop improvement
- Explores plant–microbe and plant–insect interactions affecting plant defense and productivity
- Covers animal types, pharmacogenomics, human tissue banking, and the molecular prognosis of ailments reminiscent of cervical melanoma, weight problems, and diabetes
- Examines the molecular facets of viral ailments, creation of commercial commodities utilizing viral biotechnology, and biotechnological makes use of of magnetic nanoparticles
- Describes using biotechnology within the nutrition, chemical, pharmaceutical, environmental conservation, and renewable strength sectors
Applied Molecular Biotechnology: the subsequent new release of Genetic Engineering serves as a springboard for brand new discoveries in molecular biology and its functions. hence, this e-book is a useful source for college kids and researchers of molecular biotechnology.
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Additional info for Applied molecular biotechnology : the next generation of genetic engineering
Most commonly used is DNA microarray though protein, peptide, and carbohydrate microarrays are also gaining importance. 1 Variants of PCR and its applications Type of PCR Description Multiplex PCR Utilizes more than one set of primers meant for simultaneous amplification of different regions of the DNA. Nested PCR It involves two sets of primers designed in such a manner that one of the primers amplifies the shorter internal fragment of the larger fragment. It is associated with the amplification of RNA sequences by first converting into double-stranded cDNA using reverse transcriptase enzyme.
Accuracy rate is high, faster, Nanooptical DNA sequencing, during incorporation cheaper, and less than 200 bases of nucleotides by DNA polymerase-based synthesis, are needed ions are produced, which are detected by semiconductor. 2 Overview of sequencing techniques Cannot be used for whole exome-sequencing, whole-genome sequencing, ChIP-Seq, and RNA-seq (Continued) The error rate is high due to noise, time consuming, 32 bp length reads 35–50 bp length reads 75 bp length reads More false positives 400 bp length reads • Requires lots of DNA (100s of ng to 1 ug) so it typically involves cloning and/or PCR • Limited throughput • High cost Disadvantage 14 Applied molecular biotechnology Oxford Nanopore Pacific Biosciences’ real-time single molecule sequencing (PacBioRS) Combined Probe Anchor hybridization and ligation (cPAL) (Complete Genomics) Method Advantage Fourth-generation sequencing It combines the single molecule sequencing of High speed of sequencing, third-generation sequencing technologies with provision for whole genome scan nanopore technology.
Pulse field gel electrophoresis DNA greater than ~40 kb length cannot be easily separated by applying constant electrical field. This problem is solved by pulsed field gel electrophoresis (PFGE) in which electric field is switched periodically between two different directions. Agarose gel electrophoresis It is frequently used for qualitative and quantitative estimation of nucleic acid, that is, DNA and RNA. Isoelectric focusing It separates the proteins in a pH gradient based on their isoelectric point (pI).