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Additional resources for Advances in Biochemical Engineering Biotechnology Metabolic Engineering

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Enhancing Precursor Supplies . . . . . . Superhosts . . . . . . . . . . . . Genomics Guided Process and Strain Improvement 8 Conclusion . . . . . . . . . . . . . . . . 48 . . . . . . . . . . . . . . . . . . . . . . . 39 40 42 46 47 References . . . . . . . . . . . . . . . . . . 48 Advances in Biochemical Engineering/ Biotechnology, Vol. 73 Managing Editor: Th. Scheper © Springer-Verlag Berlin Heidelberg 2001 32 R. McDaniel et al.

Fu H, Ebert-Khosla S, Hopwood DA, Khosla C (1994) Relaxed specificity of the oxytetracycline polyketide synthase for an acetate primer in the absence of a malonamyl primer. J Am Chem Soc 116:6443–6444 62. Kao CM, Katz L, Khosla C (1994) Engineered biosynthesis of a complete macrolactone in a heterologous host. Science 265:509–512 63. Tang L, Fu H, Betlach M, McDaniel R (1999) Elucidating the mechanism of chain termination switching in the picromycin/methymycin polyketide synthase. Chem Biol 6: 553–558 Process Development and Metabolic Engineering 51 64.

This was recently addressed by examining the activity of 6-deoxyerythronolide B synthase (DEBS) isolated from an overproducing S. erythraea strain. The production levels of DEBS from this strain and DEBS isolated from the wild-type strain were similar when expressed in a non-overproducing host (R. McDaniel, unpublished). This suggests that the factors contributing to overproduction lie predominantly in expression levels and/or precursor availability and that PKS optimization may not be important.

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