Download Advanced Techniques in Soil Microbiology by R. Jeewon, K. D. Hyde (auth.), Prof. Dr. Ajit Varma, Prof. PDF

By R. Jeewon, K. D. Hyde (auth.), Prof. Dr. Ajit Varma, Prof. Dr. Ralf Oelmüller (eds.)

"Advanced ideas in Soil Microbiology" provides quite a lot of biotechnological equipment for program in soil microbiology research. those comprise all crucial equipment regarding molecular biology, immunology, microbiology, and structural biology, reminiscent of transcriptome research, RNAi know-how, molecular matchmaking, RAPD, T-RFLP and FT/MS.

The ideas and strategies were chosen with the purpose of providing useful publications for instant use within the laboratory. The structures investigated diversity from person molecules and cells to whole eukaryotic organisms, with a spotlight on micro organism, fungi, mycorrhiza, and better crops. This quantity of state of the art, perform orientated tools could be of significant use either to the first-timer and to the skilled scientist.

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Fungal Divers 17:39–55 Kirk JL, Beaudette LA, Hart M, Moutoglis P (2004) Methods of studying soil microbial diversity. J Microbiol Methods 58:169–188 Kouichi S, Yoshihisa S, Masanori S, Kazuo S (2005) A new primer for discrimination of arbuscular mycorrhizal fungi with polymerase chain reaction-denature gradient gel electrophoresis. Grassl Sci 51:179–181 Kowalchuk GA, Gerards S, Woldendorp JW (1997) Detection and characterization of fungal infections of Ammophila arenaria (Marram grass) roots by denaturing gradient gel electrophoresis of specifically amplified 18s rDNA.

The cDNA fragment coding for cyclophilin-like protein was labeled with 32P-dCTP with the Rediprime DNA labeling kit (Amersham Pharmacia Biotech) and used as a probe in the hybridization analyses of the membrane-bound nucleic acids, as described previously (Sambrook et al. 1989; Kim et al. 1999). 1 Preparation of Fungal Material Laccaria bicolor mycelium was freshly cultured on a cellophane sheet overlaid on low glucose-MMN (2% glucose) agar medium at 22 °C for 1 week, as described by Balasubramanian et al.

With the advent of many high throughput techniques that have been successfully applied to the functional analysis of genes from many organisms, it is now possible to apply similar strategies to study the various aspects of the mycorrhizal symbiosis. In this chapter, we describe protocols applied to study of ectomycorrhizal symbiosis leading to: (1) Yeast two-hybrid methods to determine the interactions of signaling proteins and signaling cascades and (2) transformation system for gene replacement and functional genomic analysis of ectomycorrhizal symbiosis.

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